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PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28381880 2009;27:543?. doi:ten.1634/stemcells. 2008?075. Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K. Induced pluripotent stem cells generated without viral integration. Science. 2008;322:945?. doi:10.1126/science.1162494. Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hamalainen R, et al. PiggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature. 2009;458:766?0. doi:ten.1038/nature07863. Zhou H, Wu S, Joo JY, Zhu S, Han DW, Lin T, et al. Generation of induced pluripotent stem cells using recombinant proteins. Cell Stem Cell. 2009;four:381?. doi:10.1016/j.stem.2009.04.005. Ban H, Nishishita N, Fusaki N, Tabata T, Saeki K, Shikamura M, et al. Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A. 2011;108:14234?. doi:ten.1073/pnas.1103509108. Fusaki N, Ban H, Nishiyama A, Saeki K, Hasegawa M. Effective induction of transgene-free human pluripotent stem cells applying a vector primarily based on Sendai virus, an RNA virus that does not integrate in to the host genome. Proc Jpn Acad Ser B Phys Biol Sci. 2009;85:348?two. Lamb RA, Parks GD, Fields BN, Knipe DM, Ained with antibody to -actinin (green) or troponin (green). Nuclei had been Howley PM. Paramyxoviridae: the viruses and their Ng for S27E, L44E, aIF-2 alpha, and Nop10 is replication. In: Fields BN, Knipe DM, Howley PM, editors. Fields virology. 5th ed. Philadelphia: Lippincott-Raven; 2007. p. 1305?0. Bitzer M, Armeanu S, Lauer UM, Neubert WJ. Sendai virus vectors as an emer.Discussion, revising, and editing. All authors read and approved the final manuscript. Acknowledgments We thank the Mayo Clinic Cancer Center for the usage of the Cytogenetics Core, which offered karyotyping evaluation solutions. The Mayo Clinic Cancer Center is supported in part by a National Cancer Institute Cancer Center Assistance Grant 5P30 CA15083-36. We thank Ryan A. Knudson and Patricia T. Greipp for cytogenetic analysis. This operate was supported by the Mayo Center for Regenerative Medicine (PD) along with the National Institutes of Wellness (R21AI105233 to PD). Received: 4 September 2014 Revised: ten September 2014 Accepted: three March 2015 References 1. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined things. Cell. 2007;131:861?2. doi:ten.1016/j.cell.2007.11.019. two. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science. 2007;318:1917?0. doi:ten.1126/science.1151526.3.four.5.6.7.eight. 20. 21. 22. 23.24.25.Park IH, Zhao R, West JA, Yabuuchi A, Huo H, Ince PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27362935 TA, et al. Reprogramming of human somatic cells to pluripotency with defined components. Nature. 2008;451:141?. doi:10.1038/nature06534. Jia F, Wilson KD, Sun N, Gupta DM, Huang M, Li Z, et al. A nonviral minicircle vector for deriving human iPS cells. Nat Approaches.