Discussion, revising, and editing. All authors study and approved the final

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Okita K, Nakagawa M, Ging negative-strand RNA viral vector technique. J Gene Med. 2003;five:543?3. doi:10.1002/jgm. Hyenjong H, Ichisaka T, Yamanaka S. Effective induction of transgene-free human pluripotent stem cells utilizing a vector based on Sendai virus, an RNA virus that will not integrate into the host genome. Proc Jpn Acad Ser B Phys Biol Sci. 2009;85:348?two. Lamb RA, Parks GD, Fields BN, Knipe DM, Howley PM. Paramyxoviridae: the viruses and their replication. In: Fields BN, Knipe DM, Howley PM, editors. Fields virology. 5th ed. Philadelphia: Lippincott-Raven; 2007. p. 1305?0. Bitzer M, Armeanu S, Lauer UM, Neubert WJ. Sendai virus D for the control, restoration of SMAD4 reduces mRNA level of vectors as an emer.Discussion, revising, and editing. All authors study and authorized the final manuscript. Acknowledgments We thank the Mayo Clinic Cancer Center for the use of the Cytogenetics Core, which provided karyotyping evaluation solutions. The Mayo Clinic Cancer Center is supported in component by a National Cancer Institute Cancer Center Help Grant 5P30 CA15083-36. We thank Ryan A. Knudson and Patricia T. Greipp for cytogenetic evaluation. This work was supported by the Mayo Center for Regenerative Medicine (PD) plus the National Institutes of Well being (R21AI105233 to PD). Received: four September 2014 Revised: ten September 2014 Accepted: three March 2015 References 1. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined components. Cell. 2007;131:861?2. doi:10.1016/j.cell.2007.11.019. 2. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science. 2007;318:1917?0. doi:ten.1126/science.1151526.3.4.five.six.7.eight.9.10.11.12.13.14.15.16.17.18.19. 20. 21. 22. 23.24.25.Park IH, Zhao R, West JA, Yabuuchi A, Huo H, Ince PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27362935 TA, et al. Reprogramming of human somatic cells to pluripotency with defined things. Nature. 2008;451:141?. doi:10.1038/nature06534. Jia F, Wilson KD, Sun N, Gupta DM, Huang M, Li Z, et al. A nonviral minicircle vector for deriving human iPS cells. Nat Methods. 2010;7:197?. doi:ten.1038/nmeth.1426. Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K. Virus-free induction of pluripotency and subsequent excision of reprogramming aspects. Nature. 2009;458:771?. doi:ten.1038/nature07864. Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S. Generation of mouse induced pluripotent stem cells without the need of viral vectors. Science. 2008;322:949?3. doi:ten.1126/science.1164270. Sommer CA, Stadtfeld M, Murphy GJ, Hochedlinger K, Kotton DN, Mostoslavsky G. Induced pluripotent stem cell generation working with a single lentiviral stem cell cassette. Stem Cells. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28381880 2009;27:543?. doi:ten.1634/stemcells. 2008?075. Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K. Induced pluripotent stem cells generated without having viral integration. Science. 2008;322:945?. doi:ten.1126/science.1162494. Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hamalainen R, et al. PiggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature. 2009;458:766?0. doi:10.1038/nature07863.